논문검색
Author Suzy Kim, Hong Gyun Wu, Jin Hee Shin, Hye Jin Park, In Ah Kim, Il Han Kim
Place of duty 1Department of Radiation Oncology, The Catholic University of Korea College of Medicine, 2Department of Radiation Oncology, Seoul National University College of Medicine, 3Cancer Research Institute, Seoul National University College of Medicine, 4Institute of Radiation Medicine, Medical Research Center, Seoul National University, 5Department of Radiation Oncology, Bundang Seoul National University Hospital.
Title Enhancement of Radiation Effects by Flavopiridol in Uterine Cervix Cancer Cells
Publicationinfo Cancer Res Treat. 2005 Jun; 037(03): 191-195.
Key_word Flavopiridol,Radiation,Uterine cervix cancer
Full-Text
Abstract Purpose: To determine the effects of combinations of radiation and flavopiridol, an inhibitor of cyclin-dependent kinases and global transcription, in a human uterine cervix cancer cell line. Materials and Methods: Human uterine cervix cancer cells (HeLa), cultured to the mid-log phase, were exposed to X-rays, flavopiridol, and combinations of X-rays and flavopiridol in various sequences. The end point in this study was the clonogenic survival, which was measured via clonogenic assays. In order to determine the intrinsic cytotoxicity of flavopiridol, 0, 5, 12.5, 25, 37.5, 50 and 100 nM of flavopiridol were added to cell culture media. In the combination treatment, four different schedules of flavopiridol and irradiation combinations were tested: treatment of flavopiridol for 24 hours followed by irradiation, simultaneous administration of flavopiridol and irradiation, and irradiation followed by flavopiridol (for 24 hours) at intervals of 6 and 24 hours. The fraction of cells surviving after the combination treatment with 2 Gy of radiation (SF2) was compared with that of the fraction of cells surviving after treatment with irradiation alone. Results: The cytotoxicity of flavopiridol was found to be dose-dependent, with an IC50 of 80 nM. No cytotoxic enhancements were observed when flavopiridol and radiation were administered simultaneously. Flavopiridol, administered either 24 hours before or 6 hours after irradiation, exerted no sensitizing effects on the cells. Only one protocol resulted in a radiosensitizing effect: the administration of flavopiridol 24 hours after irradiation. Conclusion: Flavopiridol enhanced the effects of radiation on a uterine cervix cancer cell line in vitro, and this enhancement was both sequence- and time-dependent. (Cancer Res Treat. 2005;37:191-195)